Understand the physics behind total internal reflection and why light can become completely trapped inside a medium. This video explains the conditions required for total internal reflection, the role ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...

A beam transformed: Optical transforms like tight focussing and total internal reflection, which gives rise to an evanescent field, can lead to out-of-plane polarization components and transverse spin ...

1. The reflection of light from the surfaces of many kinds of leaves has been measured by means of a direct reading spectrophotometer, the measurements being made normal (that is, at an angle of 90⚬ ...

In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...